Benjamin Gaston, MD
Children’s Lung Foundation Professor of Pediatric Pulmonology
Case Western Reserve University School of Medicine
Chief, Division of Pulmonary Diseases
UH Rainbow Babies and Children’s Hospital
My laboratory studies cysteine modifications downstream of nitric oxide synthase (NOS) activation. These are relevant to cystic fibrosis (CF), asthma, pulmonary arterial hypertension and apnea. I have nearly two decades’ experience with S-nitrosothiol assays, and state-of-the-art technology for detecting cysteine modifications downstream of NOS activation. I also have extensive experience studying NOS in cell and molecular biology.
Palmer, Lisa Ann PhD
Principal Investigator, TITUS Group Project 2
Associate Professor, Pediatrics and Anesthesiology
University of Virginia
Since my arrival at the University of Virginia in 1995, I have been working on the regulation of nitric oxide synthases and nitric oxide signaling pathways. As activation of any one of the nitric oxide synthases correlates with the production of protein S-nitrosothiols, my familiarity with the biological, molecular, and signaling aspects of this enzyme system will be a benefit. In addition, I am proficient in all of the technical aspects of measuring, characterizing, and modulating S-nitrosothiols in vivo, ex vivo and in vitro. I have an excellent working relationship with Dr. Zaman. In fact, we meet regularly to discuss current topics in S-nitrosothiol research, recent advances in the field, current research results and future projects. I will consult and help perform experiments, particularly in the field of molecular biology. In addition, I will review prepared manuscripts.
Lewis, Stephen John PHD
Principal Investigator, TITUS Group Project 3
Professor, Division of Pediatric Pulmonology, and Allergy/Immunology
Department of Pediatrics
Case Western Reserve University
I have over twenty years experience studying S-nitrosothiol pharmacology. I will assist Dr. Zaman in studying the metabolism of S-nitrosothiols in human airway pseudostratified epithelial cell culture.