Ammasi Periasamy, PhD
Director, TITUS Group Imaging Core
Professor and Center Director
The W.M. Keck Center for Cellular Imaging
University of Virginia
Dr. Periasamy is an internationally recognized expert in advanced light microscopy techniques, particularly in the area of molecular imaging in living cells and tissues. He is one of the pioneers in developing lifetime imaging microscopy for intracellular calcium measurement and later he developed the same methodology for protein-protein interactions (FLIM-FRET) and cancer diagnosis. He has published over 100 articles in refereed journals and book chapters. He has given over 100 invited lectures nationally and internationally. Dr. Periasamy has edited three books, Chairperson (since 2001) in organizing an annual International conference on Multiphoton Microscopy in the Biomedical Sciences through SPIE and runs a hands on training annual workshop (since 2002) on FRET Microscopy at the University of Virginia, Charlottesville during March. Dr. Periasamy is one of the elected “Fellow” member of the SPIE Optical Society.
Khalequz Zaman, PhD
Director, TITUS Group Cell Culture Core
Assistant Professor, Division of Pediatric Pulmonology, and Allergy/Immunology
Department of Pediatrics
Case Western Reserve University
Background: During my past thirteen years, I have focused on the exciting area concerning how endogenous S-nitrosothiols (SNOs) such as S-nitrosoglutathione (GSNO) up-regulate the expression, maturation and function of cystic fibrosis (CF) transmembrane conductance regulator (CFTR) in cystic fibrosis airway epithelial cells. The primary goal of our research is to define the novel molecular mechanisms by which SNOs permit CFTR maturation and trafficking to cell surface of human airway epithelial cells. These mechanistic insights will allow GSNO and related SNO compounds to be optimized as agents for CF therapy.
Role in TITUS Group: In order to perform biochemical and molecular studies regarding S-nitrosothiols signaling in vitro, each of the projects make extensive use of primary cell cultures. For this reason, we have established a cell culture core and I am responsible for the operation of the cell core facility. Overall, cell culture core provide:
- Primary human airway pseudostratified columnar epithelium grown at air liquid interface from normal subjects and from subjects with cystic fibrosis (Project 1).
- Primary human pulmonary microvascular endothelial cells and mouse endothelial cells (Project 2).
- Primary adrenal medulla cells (Project 3).
- In addition, the cell culture core also maintain active cultures of many types of epithelial cell lines as well as maintain frozen stocks of a larger cell library.